Composite

Part:BBa_I712037:Design

Designed by: Katja Kolar   Group: iGEM07_Ljubljana   (2007-10-24)


CMVp - CD4 - CUb - mCherry - NLS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1263
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2051
    Illegal BamHI site found at 2889
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1876
    Illegal NgoMIV site found at 1925
    Illegal AgeI site found at 2006
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1710
    Illegal SapI.rc site found at 798


Design Notes

The construct was cloned into pSB1AK3 vector with terminator.

Source

For more information please check basic parts.

References